Chromatography of histones.
نویسنده
چکیده
منابع مشابه
Rapid Purification of Recombinant Histones
The development of methods to assemble nucleosomes from recombinant histones decades ago has transformed chromatin research. Nevertheless, nucleosome reconstitution remains time consuming to this day, not least because the four individual histones must be purified first. Here, we present a streamlined purification protocol of recombinant histones from bacteria. We termed this method "rapid hist...
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The interaction of sodium n-dodecyl sulphate (SDS) with histones in phosphate buffer pH 6.4 has been studied spectroscopically and by equilibrium dialysis. The enthalpies of interaction and the spectroscopic data suggest different structural stability for histones-SDS interaction. The linear relation between enthaly and absorbtion was determined by the equation of m= , where m is the sta...
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Five major histone proteins have been extracted from chromatin isolated from purified nuclei of the fungus, Aspergillus nidulans. These proteins had chromatographic properties which were similar to reference calf thymus histones and were purified to electrophoretic homegeneity by gel chromatography of Bio-Gel P10, Bio-Gel P60, and Sephadex G-100. Electrophoresis of these proteins in three diffe...
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The histone extracted from calf thymus glands is a complex system of proteins, which can be fractionated by chromatography on carboxymethyl cellulose columns into three principal fractions (1) very lysine-rich, (2) moderately lysine-rich, (3) arginine-rich. When examined by starch gel chromatography each of these gives more than one band. Methods have been devised for further separation of the ...
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عنوان ژورنال:
- The Biochemical journal
دوره 66 4 شماره
صفحات -
تاریخ انتشار 1957